The Iranian researchers at Tarbiat Modarres University managed to introduce a method for breast cancer treatment by using nano-balls in photothermal imaging.
Elaborating on the mechanism of photothermal imaging or heat therapy by nano-balls, Dr. Farzaneh Sheikholeslami said, "Silica core is used as a base for gold particles in this method. An antibody designed against the main cancer cell antigen (HER-2) attaches to this complex."
"After interactions between antibody and antigen, the mentioned complex attaches to cancer cell surface and when it is irradiated by laser beams at wavelengths ranged from 750 to 1100 nanometers coming from outside, excited gold particles move to higher levels and release the received energy over return to their initial level resulting in cancer cells (SK-Br-3) death while witness cells (HelaS3) don’t get hurt and stay safe,” she added.
Asked about the results of the research, Sheikholeslami continued saying that," We were anticipating that a nano-ball attached to SR-87 nanobody only attached to SKBr3 cells which had HER-2 receptor and revealed its fatal effect just to these cancer cells which, practically, turned out to be true."
Noting that taken images and MTT Assay showed that only 19% of SKBr3 cells survived after laser irradiation (implying that 81% of them were destroyed), she reiterated, "89% of HElaS3 cells survived because they didn’t have any HER-2 receptor on their surfaces to which nanobodies and their associated nano-balls could attach and destroy the cells. A slight reduction in the number of these cells (11%) results from repeated washings, adding or subtracting reagents and changing medium causing cells detachment and loss."
It is notable that the mentioned nanobody sequencing alignment is registered to this researcher at Gene Bank.
A part of the present research details are available at LABMEDICINE, volume 4, pages 69-76, 2010.
Source: Iran Nanotechnology Initiative Council news service